patients, 76 (57%) were p16-positive and 57 (43%) were p16-negative. ECS status did not correlate with DSS among p16-positive or p16-negative OPC patients. CONCLUSION: ECS was not associated with worse DSS in p16-positive or p16-negative OPC patients. Adverse prognostic value of ECS in OCC patients was confirmed. Cancer 2013;119:3302-8. The p16-positive slides were carefully evaluated according to the four criteria of nuclear score proposed by Wentzensen et al 17. These four criteria are: Increased nuclear/cytoplasmic ratio, Chromatin granule, Irregular nuclear shape, and Anisonucleosis. p16-positive cells without any further sign of nuclear alterations were given a score of 1. HPV is short for human papillomavirus. HPVs are a large group of related viruses. Each virus in the group is given a number, which is called an HPV type. Most HPV types cause warts on the skin, such as on the arms, chest, hands, or feet. Other types are found mainly on the body's mucous membranes. Mean age of the patients involved in the study was 48.9 years. Most of the patients presented at stage T2 with a high mean ki67 index i.e. 46.9%. 42.7% of cases had nodal metastasis. Seventy-one percent (98 cases) of cases showed positive p16 expression, whereas 24.8% (34 cases) were negative and 3.6% (5 cases) showed focal positive p16 Usual type endocervical adenocarcinomas, in contrast, are usually negative for ER, negative for vimentin, positive for CEA, and diffusely positive for p16. If the carcinoma in question demonstrates high-grade cytologic features (as with serous or undifferentiated types), the utility of p16 is limited, as high-grade tumors can express diffuse Human papillomavirus (HPV)-mediated oropharyngeal (p16+) cancer staging refers to TNM staging of squamous cell carcinomas of the oropharynx that test positive for p16, an immunohistochemical proxy for HPV infection. Nodal metastases of p16+ squamous cell carcinoma without an identified primary tumor are also staged under this system. The phrase "endocervical cells present" simply means that your doctor sampled cells from the inside of your cervix during the Pap smear. The phrase "squamous metaplastic cells present Endometrial carcinomas are heterogeneous tumors that require accurate diagnosis and classification. This article reviews the current criteria and challenges for interpreting p53 immunohistochemistry in endometrial carcinomas, and proposes a practical algorithm to improve reproducibility and clinical utility. Learn more about the role of p53 in endometrial carcinogenesis and prognosis. CDKN2A is a tumor suppressor gene located on chromosome 9p21 that encodes the cell cycle inhibitor protein p16 [].Genetic alterations of this gene are frequently observed in various types of human cancers [1-3].With regard to brain tumors and prognosis, homozygous deletion of the CDKN2A gene has been reported to be associated with shortened survival in isocitrate dehydrogenase (IDH)-mutant It is known that breast cancer may express p16 positivity from 10-55% [ 10 ]. There are no data in metaplastic breast cancer for p16 positivity to our knowledge. Metaplastic breast cancer is an aggressive subtype of breast cancer and may be frequently be axillary node negative. According to Hennessy although mostly triple negative they resemble g2c8.